diff --git a/src/lib/spinel/radio_spinel_impl.hpp b/src/lib/spinel/radio_spinel_impl.hpp
index d11a2e568..e563d00ac 100644
--- a/src/lib/spinel/radio_spinel_impl.hpp
+++ b/src/lib/spinel/radio_spinel_impl.hpp
@@ -248,7 +248,9 @@ void RadioSpinel<InterfaceType, ProcessContextType>::Init(bool aResetRadio,
 
         if (aRestoreDatasetFromNcp)
         {
+#if !OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
             exitCode = (RestoreDatasetFromNcp() == OT_ERROR_NONE) ? OT_EXIT_SUCCESS : OT_EXIT_FAILURE;
+#endif
         }
 
         DieNow(exitCode);
@@ -412,6 +414,7 @@ exit:
     return error;
 }
 
+#if !OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
 template <typename InterfaceType, typename ProcessContextType>
 otError RadioSpinel<InterfaceType, ProcessContextType>::RestoreDatasetFromNcp(void)
 {
@@ -429,6 +432,7 @@ exit:
     Instance::Get().template Get<SettingsDriver>().Deinit();
     return error;
 }
+#endif
 
 template <typename InterfaceType, typename ProcessContextType>
 void RadioSpinel<InterfaceType, ProcessContextType>::Deinit(void)
@@ -585,6 +589,7 @@ exit:
     LogIfFail("Error processing response", error);
 }
 
+#if !OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
 template <typename InterfaceType, typename ProcessContextType>
 otError RadioSpinel<InterfaceType, ProcessContextType>::ThreadDatasetHandler(const uint8_t *aBuffer, uint16_t aLength)
 {
@@ -738,6 +743,7 @@ otError RadioSpinel<InterfaceType, ProcessContextType>::ThreadDatasetHandler(con
 exit:
     return error;
 }
+#endif // #if !OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE
 
 template <typename InterfaceType, typename ProcessContextType>
 void RadioSpinel<InterfaceType, ProcessContextType>::HandleWaitingResponse(uint32_t          aCommand,
@@ -2268,8 +2274,12 @@ void RadioSpinel<InterfaceType, ProcessContextType>::RestoreProperties(void)
                          sizeof(otMacKey)));
     }
 
-    SuccessOrDie(Instance::Get().template Get<Settings>().ReadNetworkInfo(networkInfo));
-    SuccessOrDie(Set(SPINEL_PROP_RCP_MAC_FRAME_COUNTER, SPINEL_DATATYPE_UINT32_S, networkInfo.GetMacFrameCounter()));
+    if (mInstance != nullptr)
+    {
+        SuccessOrDie(static_cast<Instance *>(mInstance)->template Get<Settings>().ReadNetworkInfo(networkInfo));
+        SuccessOrDie(
+            Set(SPINEL_PROP_RCP_MAC_FRAME_COUNTER, SPINEL_DATATYPE_UINT32_S, networkInfo.GetMacFrameCounter()));
+    }
 
     for (int i = 0; i < mSrcMatchShortEntryCount; ++i)
     {