diff --git a/src/posix/platform/spi_interface.cpp b/src/posix/platform/spi_interface.cpp
index f3078b46c..2040cd58e 100644
--- a/src/posix/platform/spi_interface.cpp
+++ b/src/posix/platform/spi_interface.cpp
@@ -326,7 +326,7 @@ uint8_t *SpiInterface::GetRealRxFrameStart(uint8_t *aSpiRxFrameBuffer, uint8_t a
     uint8_t       *start = aSpiRxFrameBuffer;
     const uint8_t *end   = aSpiRxFrameBuffer + aAlignAllowance;
 
-    for (; start != end && start[0] == 0xff; start++)
+    for (; start != end && ((start[0] == 0xff) || (start[0] == 0x00)); start++)
         ;
 
     aSkipLength = static_cast<uint16_t>(start - aSpiRxFrameBuffer);
@@ -469,7 +469,7 @@ otError SpiInterface::PushPullSpi(void)
         DieNow(OT_EXIT_FAILURE);
     }
 
-    // Account for misalignment (0xFF bytes at the start)
+    // Account for misalignment (0xFF or 0x00 bytes at the start)
     spiRxFrame = GetRealRxFrameStart(spiRxFrameBuffer, mSpiAlignAllowance, skipAlignAllowanceLength);
 
     {