diff --git a/src/core/diags/README.md b/src/core/diags/README.md
index 7af2f6084..550223d02 100644
--- a/src/core/diags/README.md
+++ b/src/core/diags/README.md
@@ -80,11 +80,12 @@ Done
 
 ### diag frame
 
-Usage: `diag frame [-s] <frame>`
+Usage: `diag frame [-c] [-s] <frame>`
 
 Set the frame (hex encoded) to be used by `diag send` and `diag repeat`. The frame may be overwritten by `diag send` and `diag repeat`.
 
-Specify `-s` to skip security processing in radio layer.
+- Specify `-s` to indicate that tx security is already processed so that it should be skipped in the radio layer.
+- Specify `-c` to enable CSMA/CA for this frame in the radio layer.
 
 ```bash
 > diag frame 11223344
diff --git a/src/core/diags/factory_diags.cpp b/src/core/diags/factory_diags.cpp
index 1c1a28c28..0ae249fbc 100644
--- a/src/core/diags/factory_diags.cpp
+++ b/src/core/diags/factory_diags.cpp
@@ -236,15 +236,25 @@ Error Diags::ProcessFrame(uint8_t aArgsLength, char *aArgs[])
     Error    error             = kErrorNone;
     uint16_t size              = OT_RADIO_FRAME_MAX_SIZE;
     bool     securityProcessed = false;
+    bool     csmaCaEnabled     = false;
 
-    if (aArgsLength >= 1)
+    while (aArgsLength > 1)
     {
         if (StringMatch(aArgs[0], "-s"))
         {
             securityProcessed = true;
-            aArgs++;
-            aArgsLength--;
         }
+        else if (StringMatch(aArgs[0], "-c"))
+        {
+            csmaCaEnabled = true;
+        }
+        else
+        {
+            ExitNow(error = kErrorInvalidArgs);
+        }
+
+        aArgs++;
+        aArgsLength--;
     }
 
     VerifyOrExit(aArgsLength == 1, error = kErrorInvalidArgs);
@@ -254,6 +264,7 @@ Error Diags::ProcessFrame(uint8_t aArgsLength, char *aArgs[])
     VerifyOrExit(size >= OT_RADIO_FRAME_MIN_SIZE, error = kErrorInvalidArgs);
 
     ResetTxPacket();
+    mTxPacket->mInfo.mTxInfo.mCsmaCaEnabled       = csmaCaEnabled;
     mTxPacket->mInfo.mTxInfo.mIsSecurityProcessed = securityProcessed;
     mTxPacket->mLength                            = size;
     mIsTxPacketSet                                = true;
diff --git a/tests/scripts/expect/cli-diags.exp b/tests/scripts/expect/cli-diags.exp
index 3f26628d0..6f2a3f192 100755
--- a/tests/scripts/expect/cli-diags.exp
+++ b/tests/scripts/expect/cli-diags.exp
@@ -159,6 +159,13 @@ send "diag send 1\n"
 expect "length 0x3"
 expect "Done"
 
+send_user "send frame with CSMA/CA enabled\n"
+send "diag frame -c 112233\n"
+expect "Done"
+send "diag send 1\n"
+expect "length 0x3"
+expect "Done"
+
 send "diag repeat stop\n"
 expect "Done"