diff --git a/src/core/instance/instance.cpp b/src/core/instance/instance.cpp
index 4a0bcfea3..2c076ed27 100644
--- a/src/core/instance/instance.cpp
+++ b/src/core/instance/instance.cpp
@@ -51,7 +51,7 @@ OT_DEFINE_ALIGNED_VAR(gInstanceRaw, sizeof(Instance), uint64_t);
 
 #endif
 
-#if OPENTHREAD_CONFIG_MULTIPLE_STATIC_INSTANCE_ENABLE
+#if OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_ENABLE && OPENTHREAD_CONFIG_MULTIPLE_STATIC_INSTANCE_ENABLE
 
 #define INSTANCE_SIZE_ALIGNED OT_ALIGNED_VAR_SIZE(sizeof(ot::Instance), uint64_t)
 #define MULTI_INSTANCE_SIZE (OPENTHREAD_CONFIG_MULTIPLE_INSTANCE_NUM * INSTANCE_SIZE_ALIGNED)