diff --git a/.github/workflows/otbr.yml b/.github/workflows/otbr.yml
index 1a352c800..d20d56231 100644
--- a/.github/workflows/otbr.yml
+++ b/.github/workflows/otbr.yml
@@ -105,7 +105,14 @@ jobs:
     runs-on: ubuntu-20.04
     strategy:
       matrix:
-        otbr_mdns: ["mDNSResponder", "avahi"]
+        include:
+          - otbr_mdns: "mDNSResponder"
+            otbr_trel: 0
+          - otbr_mdns: "mDNSResponder"
+            otbr_trel: 1
+          - otbr_mdns: "avahi"
+            otbr_trel: 0
+    name: BR (${{ matrix.otbr_mdns }}, TREL=${{matrix.otbr_trel}})
     env:
       REFERENCE_DEVICE: 1
       VIRTUAL_TIME: 0
@@ -123,6 +130,7 @@ jobs:
     - name: Build OTBR Docker
       env:
         GITHUB_TOKEN: "${{ secrets.GITHUB_TOKEN }}"
+        TREL: ${{ matrix.otbr_trel }}
       run: |
         ./script/test build_otbr_docker
     - name: Bootstrap
diff --git a/script/test b/script/test
index bae466ae4..b9a5ee442 100755
--- a/script/test
+++ b/script/test
@@ -50,6 +50,7 @@ readonly INTER_OP_BBR="${INTER_OP_BBR:-1}"
 
 readonly OT_COREDUMP_DIR="${PWD}/ot-core-dump"
 readonly FULL_LOGS=${FULL_LOGS:-0}
+readonly TREL=${TREL:-0}
 
 build_simulation()
 {
@@ -277,12 +278,18 @@ do_build_otbr_docker()
         "-DOT_NETDATA_PUBLISHER=ON"
         "-DOT_SLAAC=ON"
         "-DOT_SRP_CLIENT=ON"
-        "-DOT_TREL=OFF"
         "-DOT_FULL_LOGS=ON"
         "-DOT_UPTIME=ON"
         "-DOTBR_DUA_ROUTING=ON"
         "-DCMAKE_CXX_FLAGS='-DOPENTHREAD_CONFIG_DNSSD_SERVER_BIND_UNSPECIFIED_NETIF=1'"
     )
+
+    if [[ ${TREL} == 1 ]]; then
+        otbr_options+=("-DOT_TREL=ON")
+    else
+        otbr_options+=("-DOT_TREL=OFF")
+    fi
+
     local otbr_docker_image=${OTBR_DOCKER_IMAGE:-otbr-ot12-backbone-ci}
 
     otbrdir=$(mktemp -d -t otbr_XXXXXX)